• Jack-Jack Parr as Scooby Doo
  • Dash Parr as Shaggy
  • Bob Parr as Fred
  • Helen Parr as Daphne
  • Violet Parr as Velma
  • Sebastian (from The Little Mermaid) as Scooby-Dum
  • Lucius Best as Batman
  • Mr. Huph as Robin
  • The Principal as Uncle Nathaniel
  • Bernie as Mr. McKnight
  • Dodo (from The Little Mermaid) as Jerry Reed
  • Cogsworth (from Beauty and the Beast) as Omar
  • Mirage as Lena Dupree
  • Ursula (from The Little Mermaid) as Mrs. Cutler
  • Scuttle (from The Little Mermaid) as Detective Neville
  • Buddy Pine as Ben Ravencroft
  • The Underminer as The Phantom Virus
  • Friend Owl (from Bambi) as The Mayor of Oakhaven
  • Melody (from The Little Mermaid 2)a s Amber
  • Prince Eric (from The Little Mermaid) as Officer Wembley
  • Bomb Voyage as Mr. Beeman
  • Lumiere (from Beauty and the Beast) as Eric
  • Gaston (from Beauty and the Beast) as Bill
  • The Police Officer #1 as Laurel
  • The Police Officer #2 as Hardy
  • Maurice (from Beauty and the Beast) as Te Ferry Driver
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