Cast[]
- Mario as Peter Parker/Spider-Man
- Princess Peach as Mary Jane Watson
- Yoshi as Harry Osborn
- Diddy Kong as Henry
- Toadsworth as Uncle Ben
- Toadiko as Aunt May
- Donkey Kong as J. Jonah Jameson
- Funky Kong as Robbie Robertson
- Ludwig "Kooky" Von Koopa as Maximillian Fargas
- Lubba as Dr. Stromm
- Toadette as Betty Brant
- Roy Koopa as Bonesaw McGraw
- Waluigi as Flash Thompson
- Bowser as Norman Osborn/Green Goblin
Gallery[]
Mario as Peter Parker/Spider-Man
Princess Peach as Mary Jane Watson