Cast[]
- Pee-wee Herman - Ansi Molina
- Joe Manganiello - Lance
- Freckles - Sedusa
- Bella - Ember McLain
- Pepper - Tempest Shadow
- Miss Yvonne - Peridot
- Penny King - Marina
- Robs - Doc and Grumpy
- Towns - People of Jolly Hood
- Dawn the Meter Maid - Nora Wakeman
- Grizzly Bear Daniels - Eduardo
- Pterri - Billy Joe Cobra
- Jambi - Badlands Dan
- Janina Gavankar - Megan Williams
- Richard Riehle - Elderod
- Brian Palermo - Professor Venomus
- Josh Meyers - Master Song Sung Blue
- Yul - Herby
- Peggy Down - Pony Head
- Frank Collison - Dr. Flug
- Darryl Stephens - Trevor
- Okacha - Blue
- Anthony Sullivan - Emara
- Cooper Huckabee - Lynn Loud Sr.
- Judy - Lori Loud
- Dunston Checks - Leni Loud
- Sofia Vergara - Luna Loud
- Cowboy Curtis - Human Bunsen
- Charles Robinson - Vicky and Tootie's Dad
- Abe - Abraham Lincoln from The Adventures of Billy and Mandy
- Queen of England - Queen of Ant
- Devil Pee-wee Herman - Mandark
- Kids - Kids from The Powerpuff Girls
- Firefighters - Wander and Grim Reaper
- Policemens - Sheriff Blubs and Deputy Durland
- People of New York - People of Townsville Imaginary Friends People of Video Games People of Gravity Falls and Aliens